Manual of Immunopathological Techniques 1st Edition PDF

E-Book Description

Manual of Immunopathological Techniques 1st Edition PDF Free Download

The use of immunopathological techniques has become increasingly widespread due to their ability to not only diagnose infectious and immunodeficiency disorders, but also haematological, oncological and gastroenterological diseases.

This manual is a practical guide to immunopathological techniques for undergraduates and trainees. Beginning with an overview of immunopathology, the following chapters provide in depth discussion on different techniques for the diagnosis and management of numerous immunological disorders, including autoimmune diseases, tumours, transplants and immunohistochemistry.

Presented in bullet point format for easy reference, this useful text is enhanced by high quality photographs and tables to assist learning.

Key points

Practical guide to immunopathological techniques
Covers different techniques for diagnosis and treatment of numerous disorders
Presented in bullet point format for easy reference
Highly illustrated with clinical photographs and tables

E-Book Details

Сategories: Medicine|Immunology|Pathology
Paperback: 178 pages
Language: English
ISBN-10: 9351520846
ISBN-13: 978-9351520849

Table of Contents

Introduction

Tests for cell mediated immunity (CMI)

Lymphocytes typing by Flow Cytometry

Diagnosis of Kala-Azar by PCR technique

Test for myeloma profile and qualitative assessment of serum proteins

Agarose gel electrophoresis for qualitative assessment of serum proteins

Cellulose Acetate Electrophoresis

Agar Gel Immunoelectrophoresis for typing of myeloma

Micro Agar Gel Double Diffusion Technique

Counter Current Immunoelectrophoresis

Single Radial Immunodiffusion and Immunoturbidometric methods for the estimation of Immunoglobulins

Estimation of complement C3, C4 and hemolytic assay for serum complement

Demonstration of immune complexes and complement by indirect immuno peroxidase method

Test for autoimmune diseases

Test for Autoantibodies by ELISA technique

Acid Fuchsin Orange G in kidney biopsy for demonstration of immune complexes

Detection of tumor markers in paraffin embedded sections by immunohistochemistry

Detection of circulating immune complexes (CIC) in the serum

Demonstration of immune complexes, antibodies and complements in renal biopsy by immunofluorescence

HLA typing and Cross Match test in solid organ transplantation

HLA B27 typing by PCR method

Fluorescence in-situ hybridisation: methods and applications

Detection of cytokines (IL-6, IL-8, TNF-a)

Western Blot or Tests for detection of antibody to extractable nuclear antigen

Detection of anti-thyroglobulin antibodies by hemagglutination method

Distillation and demineralization of water

Raising of polyclonal anti human anti-IgG in rabbit
Manual of Immunopathological Techniques 1st Edition PDF Ebook

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About the Authors

UshaMD
Professor and Head, Department of Pathology; Incharge, UGC Advanced Immunodiagnostic Training & Research Centre, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India

Gyanendra Kumar SonkarPhD
Assistant Professor, Department of Biochemistry, King George’s Medical University, Lucknow, Uttar Pradesh, India

Sangeeta SinghPhD
Ex-Senior Research Fellow, UGC Advanced Immunodiagnostic Training and Research Centre, Division of Immunopathology, Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India

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