Transfusion Medicine and Hemostasis: Clinical and Laboratory Aspects 2nd Edition PDF

Contributors xix
Preface xxv
Acknowledgments xxvii
PART Iâ•… Blood Banking and Transfusion Medicine
1 Blood Banking and Transfusion Medicine – History, Industry,
and Discipline 3
Christopher D. Hillyer, MD
2 Quality Principles in Transfusion Medicine 11
Eva D. Quinley, MS, SBB, CQA (ASQ)
3 Regulatory Issues in Transfusion Medicine 21
Eva D. Quinley, MS, SBB, CQA (ASQ)
Blood Banking
4 Role of the Physician in the Blood Center 31
Christopher D. Hillyer, MD and Beth H. Shaz, MD
Donation Process
5 Blood Donor, Donation Process and Technical Aspects
of Blood Collection 37
Debra A. Kessler, RN, MS
6 Apheresis Blood Component Collections 45
Benjamin J. Greco, MD
7 Recipient-Specific Blood Donation 49
Benjamin J. Greco, MD
8 Adverse Donor Reactions 53
Benjamin J. Greco, MD and Beth H. Shaz, MD
vi Contents
Component Manufactaring
9 Component Preparation and Manufacturing 61
Christopher D. Hillyer, MD
Donor Testing
10 Serologic Testing of Donor Products 69
Beth H. Shaz, MD
11 Overview of Infectious Disease Testing 73
Christopher D. Hillyer, MD and Beth H. Shaz, MD
12 Human Immunodeficiency Virus Screening 79
Debra A. Kessler, RN, MS
13 Hepatitis B Viral Screening 85
Debra A. Kessler, RN, MS
14 Hepatitis C Virus Screening 91
Debra A. Kessler, RN, MS
15 West Nile Virus Screening 95
Debra A. Kessler, RN, MS
16 Syphilis, Human T-Cell Lymphotropic Virus, and Chagas Screening 97
Debra A. Kessler, RN, MS
17 Bacterial Detection Methods 103
Beth H. Shaz, MD and Marie-Laure Desormeaux, MD
Transfusion Medicine
18 Role of the Transfusion Service Physician 109
Beth H. Shaz, MD and Christopher D. Hillyer, MD
Testing
19 Pretransfusion Testing 117
Sadiqa Karim, MD
20 Antibody Identification 127
Sadiqa Karim, MD
21 Direct Antiglobulin Test 137
Sadiqa Karim, MD
22 Molecular DNA Based Blood Group Typing 141
Connie M. Westhoff, PhD, SBB
Antigens
23 ABO and H Blood Group System 149
Connie M. Westhoff, PhD, SBB and Beth H. Shaz, MD
Contents vii
24 Rh and RhAG Blood Group Systems 157
Connie M. Westhoff, PhD, SBB and Beth H. Shaz, MD
25 Kell and Kidd Blood Group Systems 163
Connie M. Westhoff, PhD, SBB and Beth H. Shaz, MD
26 MNS and Duffy Blood Group Systems 167
Connie M. Westhoff, PhD, SBB and Beth H. Shaz, MD
27 Lewis, I, P1PK and GLOB Blood Group Systems 171
Connie M. Westhoff, PhD, SBB and Beth H. Shaz, MD
28 Other Blood Group Systems, Collections, and Series 177
Connie M. Westhoff, PhD, SBB and Beth H. Shaz, MD
29 Human Platelet Antigens 185
Scott T. Avecilla, MD, PhD
30 HLA Antigens 191
Andromachi Scaradavou, MD
Blood Products
31 Red Blood Cell Products 199
Eldad A. Hod, MD and Christopher D. Hillyer, MD
32 Plasma Products 209
Matthew S. Karafin, MD and Christopher D. Hillyer, MD
33 Platelet Products 219
Melissa M. Cushing, MD
34 Cryoprecipitate and Fibrinogen Concentrates 227
Beth H. Shaz, MD and Christopher D. Hillyer, MD
35 Granulocyte Products 231
Suzanne A. Arinsburg, DO
36 Albumin and Related Products 237
Anne M. Winkler, MD
37 Human Immunoglobulin Preparations 243
Matthew S. Karafin, MD and Beth H. Shaz, MD
38 Rh Immune Globulin 255
Matthew S. Karafin, MD and Beth H. Shaz, MD
39 Coagulation Factor Products 259
Scott T. Avecilla, MD, PhD
Product Modifications
40 Irradiation of Blood Products 269
Richard O. Francis, MD, PhD
viii Contents
41 Leukoreduction of Blood Products 275
James C. Zimring, MD, PhD and Theresa Nester, MD
42 CMV-Safe Blood Products 279
Jennifer L. Garbaini, MD and Christopher D. Hillyer, MD
43 Frozen Blood Products 283
Cheryl A. Goss, MD
44 Washed Blood Products 287
Cheryl A. Goss, MD
45 Volume Reduced Products 291
Cassandra D. Josephson, MD
46 Pathogen Reduction Technologies 295
Susanne Marschner, PhD and Raymond P. Goodrich, PhD
Specific Clinical Settings
47 Neonatal and Pediatric Transfusion Medicine 301
Jeanne E. Hendrickson, MD and Cassandra D. Josephson, MD
48 Perinatal Transfusion Medicine 307
Beth H. Shaz, MD
49 Autoimmune Hemolytic Anemias 317
Devika Lal, MD and Christopher D. Hillyer, MD
50 Transfusion Management in Patients with Hemoglobinopathies 327
Patricia A. Shi, MD
51 Transfusion of Patients Undergoing Hematopoietic Stem Cell
and Solid Organ Transplantation 337
Jeanne E. Hendrickson, MD
52 Transfusion Management of Patients Receiving Antithrombotic
Therapy 343
Scott T. Avecilla, MD, PhD
53 Blood Transfusion in Economically Restricted and Developing
Countries 349
Heather A. Hume, MD, FRCPC
54 Management of Patients Who Refuse Blood Transfusion 357
Jeanne E. Hendrickson, MD
55 Platelet Transfusion Refractory Patients 361
Jeanne E. Hendrickson, MD
56 Massive Transfusion 367
Beth H. Shaz, MD and Christopher D. Hillyer, MD
57 Patient Blood Management 373
Patricia A. Shi, MD
Contents ix
Transfusion Reactions
58 Overview of Adverse Events and Outcomes Following Transfusion 383
Aleksandar M. Babic, MD, PhD and Christopher D. Hillyer, MD
59 Febrile Non-Hemolytic Transfusion Reactions 389
Irina Knezevic Maramica, MD, PhD
60 Allergic Transfusion Reactions 395
William J. Savage, MD, PhD
61 Acute Hemolytic Transfusion Reactions 401
Michael Bellone, DO and Christopher D. Hillyer, MD
62 Delayed Hemolytic Transfusion Reactions 409
Cassandra D. Josephson, MD
63 Transfusion-Associated Circulatory Overload 413
Jeanne E. Hendrickson, MD and Christopher D. Hillyer, MD
64 Transfusion-Related Acute Lung Injury 417
Jeanne E. Hendrickson, MD and Christopher D. Hillyer, MD
65 Septic Transfusion Reactions 421
Irina Knezevic Maramica, MD, PhD
66 Metabolic, Hypotensive and Other Acute Reactions and
Complications 427
Beth H. Shaz, MD
67 Post-Transfusion Purpura 433
Melissa M. Cushing, MD
68 Transfusion-Associated Graft-Versus-Host Disease 435
Richard O. Francis, MD, PhD
69 Transfusion-Related Immunomodulation 445
James C. Zimring, MD, PhD and Theresa Nester, MD
70 Iron Overload 449
Yelena Z. Ginzburg, MD and Beth H. Shaz, MD
71 Transfusion-Transmitted Diseases 453
Louis M. Katz, MD and Roger Y. Dodd, PhD
Therapeutic Apheresis and Phlebotomy
72 Overview of Therapeutic Apheresis 467
Huy P. Pham, MD and Joseph Schwartz, MD
73 Therapeutic Plasma Exchange 481
Huy P. Pham, MD and Joseph Schwartz, MD
74 Therapeutic Erythrocytapheresis 505
Huy P. Pham, MD and Joseph Schwartz, MD
x Contents
75 Therapeutic Thrombocytapheresis 509
Huy P. Pham, MD and Joseph Schwartz, MD
76 Therapeutic Leukocytapheresis and Adsorptive Cytapheresis 511
Huy P. Pham, MD and Joseph Schwartz, MD
77 Extracorporeal Photopheresis 515
Huy P. Pham, MD and Joseph Schwartz, MD
78 LDL Apheresis 521
Huy P. Pham, MD and Joseph Schwartz, MD
79 Immunoadsorption 525
Huy P. Pham, MD and Joseph Schwartz, MD
80 Therapeutic Phlebotomy 529
Yelena Z. Ginzburg, MD and Beth H. Shaz, MD
Cellular Therapy and Tissue Banking
81 Overview of Cellular Therapy 533
Rona S. Weinberg, PhD
82 Hematopoietic Progenitor Cell Products Derived from Bone
Marrow and Peripheral Blood 541
Rona S. Weinberg, PhD
83 Cord Blood Banking 549
Andromachi Scaradavou, MD
84 Regenerative Medicine 559
Marie Csete, MD, PhD
85 Tissue Banking in the Hospital Setting 565
Marie-Laure Desormeaux, MD and Cassandra D. Josephson, MD
86 Adverse Events Associated with Hematopoietic Progenitor Cell
Product Infusion 571
Anna-Sophie Rich, MD and Melissa M. Cushing, MD
87 Quality and Regulatory Issues in Cellular Therapy 577
Eva D. Quinley, MS, SBB, CQA(ASQ)
PART IIâ•… Hemostasis
88 Overview of the Coagulation System 587
Thomas C. Abshire, MD and Shawn M. Jobe, MD, PhD
Clinical Coagulation
89 Approach to the Bleeding Patient 593
Thomas C. Abshire, MD and Charles S. Abrams, MD
Contents xi
Neonatal Thrombocytopenia
90 Congenital Thrombocytopenia 601
Michele P. Lambert, MD
91 Fetal and Neonatal Alloimmune Thrombocytopenia 609
Catherine E. McGuinn, MD, W. Beau Mitchell, MD
and James B. Bussel, MD
92 Acquired Neonatal Thrombocytopenia 615
Catherine E. McGuinn, MD, W. Beau Mitchell, MD
and James B. Bussel, MD
Inherited Platelet Function Disorders
93 Bernard-Soulier Syndrome and Other GPIb-IX–V Related
Receptor Defects 621
Shawn M. Jobe, MD, PhD
94 Glanzmann Thrombasthenia 625
Shawn M. Jobe, MD, PhD
95 Platelet Storage Granule Defects 627
Shawn M. Jobe, MD, PhD
96 Failure to Release and Aspirin-like Defects 631
Shawn M. Jobe, MD, PhD
Immune Thrombocytopenia
97 Acute (Childhood) Immune Thrombocytopenia 635
W. Beau Mitchell, MD and Carolyn M. Bennett, MD
98 Chronic Immune Thrombocytopenia 641
W. Beau Mitchell, MD and Carolyn M. Bennett, MD
99 Drug-Induced Thrombocytopenia 647
W. Beau Mitchell, MD and Carolyn M. Bennett, MD
100 Heparin-Induced Thrombocytopenia 651
Adam C. Cuker, MD, MS
101 Autoimmune Lymphoproliferative Syndrome 663
Michael A. Briones, DO
Immune Medicated Coagulopathies
102 Hemolytic Uremic Syndrome 669
Michael A. Briones, DO
103 Thrombotic Thrombocytopenic Purpura 675
Christine L. Kempton, MD, MSc
104 Antiphospholipid Syndrome 681
Wendy Lim, MD, MSc
xii Contents
Congenital Bleeding Disorders
105 von Willebrand Disease 691
Thomas C. Abshire, MD and Charles S. Abrams, MD
106 Hemophilia A 699
Amy L. Dunn, MD
107 Hemophilia B 705
Amy L. Dunn, MD
108 Congenital Disorders of Fibrinogen 711
Shannon L. Meeks, MD
109 Factor XIII, α2-Antiplasmin and Plasminogen Activator Inhibitor-1
Deficiencies 715
Shannon L. Meeks, MD
110 Factor XI Deficiency 719
Thomas C. Abshire, MD and Charles S. Abrams, MD
111 Factor VII Deficiency 723
Shannon L. Meeks, MD
112 Factor II, Factor V and Factor X Deficiencies 725
Shannon L. Meeks, MD
Acquired Bleeding Disorders
113 Bleeding Disorders in Pregnancy 729
Christine L. Kempton, MD, MSc
114 Vascular Bleeding Disorders 737
Michael A. Briones, DO
115 Bleeding Risks with Liver Disease 743
Thomas C. Abshire, MD and Charles S. Abrams, MD
116 Bleeding Risks with Vitamin K Deficiency 747
Thomas C. Abshire, MD and Charles S. Abrams, MD
117 Bleeding Risks with Cardiac Disease 753
Thomas C. Abshire, MD and Charles S. Abrams, MD
118 Bleeding Risks with Renal Disease 759
Thomas C. Abshire, MD and Charles S. Abrams, MD
119 Bleeding Disorders in Cancer Patients 763
Joshua M. Ruch, MD and Suman L. Sood, MD
120 Disseminated Intravascular Coagulopathy 775
Amy L. Dunn, MD
121 Acquired Coagulation Factor Inhibitors 781
Christine L. Kempton, MD, MSc
Contents xiii
Laboratory Testing of Coagulation
122 Overview of Purposes of Hemostasis Testing and Common
Sources of Error 787
Mikhail Roshal, MD, PhD
Screening Tests
123 Thrombin Time and Fibrinogen Determination 793
Mikhail Roshal, MD, PhD
124 Prothrombin Time 799
Mikhail Roshal, MD, PhD
125 Activated Partial Thromboplastin Time 805
Mikhail Roshal, MD, PhD
Laboratory Assessment of Coagulation Disorders
126 Mixing Studies 809
Connie H. Miller, PhD
127 Coagulation Factor Testing 811
Connie H. Miller, PhD
128 Specific Factor Inhibitor Testing 815
Connie H. Miller, PhD
129 Laboratory Diagnosis of Lupus Anticoagulant and Anti-Phospholipid
Antibodies 819
Mikhail Roshal, MD, PhD
130 Laboratory Diagnosis of Inherited von Willebrand Disease 825
Connie H. Miller, PhD
131 Laboratory Diagnosis of Acquired von Willebrand Syndrome 833
Connie H. Miller, PhD
132 Laboratory Assessment of Treatment of von Willebrand Disease 835
Connie H. Miller, PhD
133 Measurement of Platelet Count, Mean Platelet Volume and
Reticulated Platelets 837
Mikhail Roshal, MD, PhD
134 Platelet Function Analyzer 843
Connie H. Miller, PhD
135 Platelet Aggregation Studies 845
Connie H. Miller, PhD
136 Laboratory Diagnosis of Platelet Function Defects 849
Connie H. Miller, PhD
xiv Contents
137 Antiplatelet Therapy Monitoring 855
Connie H. Miller, PhD and Mikhail Roshal, MD, PhD
138 Laboratory Diagnosis of Heparin-Induced Thrombocytopenia 861
Anne M. Winkler, MD
139 Laboratory Evaluation of Thrombotic Thrombocytopenic Purpura 867
Christine L. Kempton, MD, MSc
140 Fibrinolytic Testing 871
Wayne L. Chandler, MD
141 Laboratory Evaluation of Factor XIII Deficiency 875
Mikhail Roshal, MD, PhD
142 Laboratory Techniques in Fibrinolysis Testing 881
Mikhail Roshal, MD, PhD
Tests for Hypercoagulable State
143 Laboratory Evaluation of Long-Term Thrombophilic Disorders 887
Mikhail Roshal, MD, PhD
144 Antithrombin Testing 893
Mikhail Roshal, MD, PhD
145 Protein C Deficiency Evaluation 899
Mikhail Roshal, MD, PhD
146 Protein S Deficiency Evaluation 905
Mikhail Roshal, MD, PhD
147 Testing for Activated Protein C Resistance 911
Anne M. Winkler, MD
148 Molecular Testing for Factor V Leiden and Prothrombin Gene
Mutations in Inherited Thrombophilia 915
Jiong Yan, MD, PhD and Hanna Rennert, PhD
149 Laboratory Management of Disseminated Intravascular
Coagulation 921
Grace F. Monis, MD, PhD and Morayma Reyes, MD, PhD
150 Circulating Microparticles 925
Florencia G. Jalikis, MD and Morayma Reyes, MD, PhD
Laboratory Support of Anticoagulation
151 Monitoring of Heparins, Fondaparinux, Direct Thrombin Inhibitors
and Oral Anti-Xa Medications 933
Mikhail Roshal, MD, PhD
152 Laboratory Support for Warfarin Monitoring 939
Anne M. Winkler, MD
Contents xv
153 Thrombin Generation Assays 945
Mikhail Roshal, MD, PhD
154 Thromboelastography/Thromboelastometry 949
Mikhail Roshal, MD, PhD
Abbreviations 957
General Handbook References
Transfusion Medicine and Hemostasis: Clinical and Laboratory Aspects 2nd Edition PDF Ebook

Beth H. Shaz, MD
Chief Medical Officer and Vice President, New York Blood Center, New York, New York,
USA
Clinical Assistant Professor, Department of Pathology and Laboratory Medicine, Emory
University School of Medicine, USA
Christopher D. Hillyer, MD
President and Chief Executive Officer, New York Blood Center, New York, New York, USA
Professor, Department of Medicine, Weill Cornell Medical College, USA
Mikhail Roshal, MD, PhD
Assistant Professor, Department of Pathology and Laboratory Medicine, Weill Cornell
Medical College, USA
New York-Presbyterian Hospital, New York, New York, USA
Charles S. Abrams, MD
Professor, Department of Medicine, University of Pennsylvania, USA
Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania, USA